AustralieFrV1, Main, Exploration, bibRecord, 00BB28

Towards the development of edible vaccines for human papillomavirus in bananas

Identifieur interne : 00BB28 ( Main/Exploration ); précédent : 00BB27; suivant : 00BB29

Towards the development of edible vaccines for human papillomavirus in bananas

Auteurs : J. Miller [Australie] ; D. Becker [Australie] ; B. Dugdale [Australie] ; R. Harding [Australie] ; J. Aaskov [Australie] ; I. Frazer [Australie] ; J. Dale [Australie]

Source :

Science update (Paris) [ 1159-554X ] ; 2001.

RBID : Pascal:01-0384520

Descripteurs français

Pascal (Inist)
- Comestibilité, Expression génique, Transfert génétique, Transformation génétique, Bombardement microprojectile, Transfection, Promoteur, Séquence non codante, Vecteur, Plante transgénique, Musa, Nicotiana tabacum, Papillomavirus humain, Agrobacterium tumefaciens, Papillomavirus bovin 1, Virus etch tabac, Virus mosaïque chou fleur, Banane, Vaccin, Voie orale, Protéine recombinante, Capside, Programme recherche, Résultat expérimental, Australie, Recherche développement.
Wicri :
- geographic : Australie.
- topic : Plante transgénique, Vaccin.

English descriptors

KwdEn :
- Banana, Edibility, Human papillomavirus, Musa, Research and development, Transgenic plant, Vaccine.

Abstract

The study of transgenic plants as vaccine production systems holds promise for the development of edible, oral vaccines for developing third world countries. We are developing bananas as a vaccine production system using microprojectile bombardment transformation and novel promoters. Further, we have engineered tobacco plants to express the coding region for the bovine papillomavirus (BPV) L1 protein (L1). The recombinant constructs contain the transgene under control of the cauliflower mosaic virus 35S promoter (CaMV35S) and the 5' untranslated region (UTR) of tobacco etch virus (TEV). Plants were transformed by Agrobacterium tumefaciens-mediated transformation of leaf discs and culture on selective media. Here we present analysis of plants transformed with BPV L1. Northern blot analysis indicated that BPV L1 mRNA of the appropriate molecular weight was produced in tissues of transformed plants, however recombinant protein was not detected in leaves or seeds of transgenic plants. In vitro translation of the BPV L1 coding region in wheat germ extracts was demonstrated, indicating that BPV Ll mRNA is translated in a plant system.

Affiliations:

Australie

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 005B22
to stream PascalFrancis, to step Curation: 000635
to stream PascalFrancis, to step Checkpoint: 005632
to stream Main, to step Merge: 00C977
to stream Main, to step Curation: 00BB28

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Towards the development of edible vaccines for human papillomavirus in bananas</title>
<author><name sortKey="Miller, J" sort="Miller, J" uniqKey="Miller J" first="J." last="Miller">J. Miller</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Queensland University of Technology, Centre for Molecular Biotechnology</s1>
<s2>Brisbane</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Becker, D" sort="Becker, D" uniqKey="Becker D" first="D." last="Becker">D. Becker</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Queensland University of Technology, Centre for Molecular Biotechnology</s1>
<s2>Brisbane</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dugdale, B" sort="Dugdale, B" uniqKey="Dugdale B" first="B." last="Dugdale">B. Dugdale</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Queensland University of Technology, Centre for Molecular Biotechnology</s1>
<s2>Brisbane</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Harding, R" sort="Harding, R" uniqKey="Harding R" first="R." last="Harding">R. Harding</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Queensland University of Technology, Centre for Molecular Biotechnology</s1>
<s2>Brisbane</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Aaskov, J" sort="Aaskov, J" uniqKey="Aaskov J" first="J." last="Aaskov">J. Aaskov</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Queensland University of Technology, Centre for Molecular Biotechnology</s1>
<s2>Brisbane</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Frazer, I" sort="Frazer, I" uniqKey="Frazer I" first="I." last="Frazer">I. Frazer</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre for Immunology and Cancer Research, Princess Alexandra Hospital</s1>
<s2>Brisbane</s2>
<s3>AUS</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dale, J" sort="Dale, J" uniqKey="Dale J" first="J." last="Dale">J. Dale</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Queensland University of Technology, Centre for Molecular Biotechnology</s1>
<s2>Brisbane</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">01-0384520</idno>
<date when="2001">2001</date>
<idno type="stanalyst">PASCAL 01-0384520 INIST</idno>
<idno type="RBID">Pascal:01-0384520</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">005B22</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000635</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">005632</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">005632</idno>
<idno type="wicri:doubleKey">1159-554X:2001:Miller J:towards:the:development</idno>
<idno type="wicri:Area/Main/Merge">00C977</idno>
<idno type="wicri:Area/Main/Curation">00BB28</idno>
<idno type="wicri:Area/Main/Exploration">00BB28</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Towards the development of edible vaccines for human papillomavirus in bananas</title>
<author><name sortKey="Miller, J" sort="Miller, J" uniqKey="Miller J" first="J." last="Miller">J. Miller</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Queensland University of Technology, Centre for Molecular Biotechnology</s1>
<s2>Brisbane</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Becker, D" sort="Becker, D" uniqKey="Becker D" first="D." last="Becker">D. Becker</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Queensland University of Technology, Centre for Molecular Biotechnology</s1>
<s2>Brisbane</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dugdale, B" sort="Dugdale, B" uniqKey="Dugdale B" first="B." last="Dugdale">B. Dugdale</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Queensland University of Technology, Centre for Molecular Biotechnology</s1>
<s2>Brisbane</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Harding, R" sort="Harding, R" uniqKey="Harding R" first="R." last="Harding">R. Harding</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Queensland University of Technology, Centre for Molecular Biotechnology</s1>
<s2>Brisbane</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Aaskov, J" sort="Aaskov, J" uniqKey="Aaskov J" first="J." last="Aaskov">J. Aaskov</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Queensland University of Technology, Centre for Molecular Biotechnology</s1>
<s2>Brisbane</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Frazer, I" sort="Frazer, I" uniqKey="Frazer I" first="I." last="Frazer">I. Frazer</name>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Centre for Immunology and Cancer Research, Princess Alexandra Hospital</s1>
<s2>Brisbane</s2>
<s3>AUS</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Dale, J" sort="Dale, J" uniqKey="Dale J" first="J." last="Dale">J. Dale</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Queensland University of Technology, Centre for Molecular Biotechnology</s1>
<s2>Brisbane</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Science update (Paris)</title>
<idno type="ISSN">1159-554X</idno>
<imprint><date when="2001">2001</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Science update (Paris)</title>
<idno type="ISSN">1159-554X</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Banana</term>
<term>Edibility</term>
<term>Human papillomavirus</term>
<term>Musa</term>
<term>Research and development</term>
<term>Transgenic plant</term>
<term>Vaccine</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Comestibilité</term>
<term>Expression génique</term>
<term>Transfert génétique</term>
<term>Transformation génétique</term>
<term>Bombardement microprojectile</term>
<term>Transfection</term>
<term>Promoteur</term>
<term>Séquence non codante</term>
<term>Vecteur</term>
<term>Plante transgénique</term>
<term>Musa</term>
<term>Nicotiana tabacum</term>
<term>Papillomavirus humain</term>
<term>Agrobacterium tumefaciens</term>
<term>Papillomavirus bovin 1</term>
<term>Virus etch tabac</term>
<term>Virus mosaïque chou fleur</term>
<term>Banane</term>
<term>Vaccin</term>
<term>Voie orale</term>
<term>Protéine recombinante</term>
<term>Capside</term>
<term>Programme recherche</term>
<term>Résultat expérimental</term>
<term>Australie</term>
<term>Recherche développement</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Australie</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Plante transgénique</term>
<term>Vaccin</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The study of transgenic plants as vaccine production systems holds promise for the development of edible, oral vaccines for developing third world countries. We are developing bananas as a vaccine production system using microprojectile bombardment transformation and novel promoters. Further, we have engineered tobacco plants to express the coding region for the bovine papillomavirus (BPV) L1 protein (L1). The recombinant constructs contain the transgene under control of the cauliflower mosaic virus 35S promoter (CaMV35S) and the 5' untranslated region (UTR) of tobacco etch virus (TEV). Plants were transformed by Agrobacterium tumefaciens-mediated transformation of leaf discs and culture on selective media. Here we present analysis of plants transformed with BPV L1. Northern blot analysis indicated that BPV L1 mRNA of the appropriate molecular weight was produced in tissues of transformed plants, however recombinant protein was not detected in leaves or seeds of transgenic plants. In vitro translation of the BPV L1 coding region in wheat germ extracts was demonstrated, indicating that BPV Ll mRNA is translated in a plant system.</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
</country>
</list>
<tree><country name="Australie"><noRegion><name sortKey="Miller, J" sort="Miller, J" uniqKey="Miller J" first="J." last="Miller">J. Miller</name>
</noRegion>
<name sortKey="Aaskov, J" sort="Aaskov, J" uniqKey="Aaskov J" first="J." last="Aaskov">J. Aaskov</name>
<name sortKey="Becker, D" sort="Becker, D" uniqKey="Becker D" first="D." last="Becker">D. Becker</name>
<name sortKey="Dale, J" sort="Dale, J" uniqKey="Dale J" first="J." last="Dale">J. Dale</name>
<name sortKey="Dugdale, B" sort="Dugdale, B" uniqKey="Dugdale B" first="B." last="Dugdale">B. Dugdale</name>
<name sortKey="Frazer, I" sort="Frazer, I" uniqKey="Frazer I" first="I." last="Frazer">I. Frazer</name>
<name sortKey="Harding, R" sort="Harding, R" uniqKey="Harding R" first="R." last="Harding">R. Harding</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 00BB28 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 00BB28 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Asie
   |area=    AustralieFrV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     Pascal:01-0384520
   |texte=   Towards the development of edible vaccines for human papillomavirus in bananas
}}

This area was generated with Dilib version V0.6.33.
Data generation: Tue Dec 5 10:43:12 2017. Site generation: Tue Mar 5 14:07:20 2024

	Serveur d'exploration sur les relations entre la France et l'Australie
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur les relations entre la France et l'Australie

Towards the development of edible vaccines for human papillomavirus in bananas

Towards the development of edible vaccines for human papillomavirus in bananas

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri